| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 381 | Tsukamurella paurometabola | AAGCGCAAGACTGGCTCA | GAGACCAAGACGTCGAGCA | 59.57 | 59.42 | 166 | 65 |
100.00%
|
100.00%
|
| Primer 382 | Tsukamurella paurometabola | AAGCGCAAGACTGGCTCA | AGACCAAGACGTCGAGCAG | 59.57 | 59.42 | 165 | 65 |
100.00%
|
100.00%
|
| Primer 383 | Tsukamurella paurometabola | ACGCCAAGATCGTCGACA | AGCGCAATTCACGGACGA | 59.04 | 60.05 | 162 | 65 |
100.00%
|
100.00%
|
| Primer 384 | Tsukamurella paurometabola | ATCGTCGACACCATGCCA | AGCGCAATTCACGGACGA | 59.02 | 60.05 | 154 | 65 |
100.00%
|
100.00%
|
| Primer 385 | Tsukamurella paurometabola | GCCAAGATCGTCGACACCA | AGCGCAATTCACGGACGA | 60.08 | 60.05 | 160 | 65 |
100.00%
|
100.00%
|
| Primer 386 | Tsukamurella paurometabola | AGATCGTCGACACCATGCC | AGCGCAATTCACGGACGA | 59.86 | 60.05 | 156 | 65 |
100.00%
|
100.00%
|
| Primer 387 | Tsukamurella paurometabola | ATCGTCGACACCATGCCAG | AGCGCAATTCACGGACGA | 60.15 | 60.05 | 154 | 65 |
100.00%
|
100.00%
|
| Primer 388 | Tsukamurella paurometabola | GATCGTCGACACCATGCCA | AGCGCAATTCACGGACGA | 60.15 | 60.05 | 155 | 65 |
100.00%
|
100.00%
|
| Primer 389 | Tsukamurella paurometabola | TCATCGCGAACTTCGGCA | CTCACCAGTGCGAATCCGA | 59.74 | 59.78 | 280 | 65 |
100.00%
|
100.00%
|
| Primer 390 | Tsukamurella paurometabola | TCATCGCGAACTTCGGCA | TCACCAGTGCGAATCCGA | 59.74 | 58.63 | 279 | 65 |
100.00%
|
100.00%
|